The Large-Scale Proteomics Research Facility Proteins@Work will consist of a core facility at Utrecht University, with smaller out-stations at the Netherlands Cancer Institute (NKI), the Erasmus Medical Centre Rotterdam and the University Medical Centre Utrecht. Proteins@Work is supported by a group of committed users, representing important life science institutes in the Netherlands, but also directly linked to the established European large-scale proteomics access facility PRIME-XS, and has strong ties with instrument manufacturers. Proteins@Work will provide open access to researchers in the life sciences from academia (universities and hospitals) and industry (pharma and nutrition, biotechnology SME’s).