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04 - Protocols
Protocols used at the Transnational Access sites of the PRIME-XS project.
Files
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PRIME-XS Protocol NPC Bradford Protein Assay.pdf
- Short Description:
- Protocol from the NPC Access Site for the determination of protein concentration using the Bradford Assay.
- Submitted On:
- 22 Jan 2013
- File Size:
- 97.75 Kb
- Downloads:
- 119
PRIME-XS Protocol NPC In Gel Digestion.pdf
- Short Description:
- Protocol from the NPC Access Site for in gel digestion of proteins using trypsin from Coomassie or Silver stained gels.
- Submitted On:
- 06 Jul 2011
- File Size:
- 109.55 Kb
- Downloads:
- 76
PRIME-XS Protocol NPC Peptide Dimethyl Labeling.pdf
- Short Description:
- Protocol from the NPC Access Site for triple dimethyl labeling of peptides in solution for quantitative proteomics analysis of multiple samples.
- Submitted On:
- 06 Jul 2011
- File Size:
- 136.26 Kb
- Downloads:
- 110
PRIME-XS Protocol NPC Cell Lysis.pdf
- Short Description:
- Protocol from the NPC Access site for the lysis of cells and tissue sample, compatible with LC-MS/MS analysis
- Submitted On:
- 12 Aug 2011
- File Size:
- 120.48 Kb
- Downloads:
- 82
PRIME-XS Protocol NPC In Solution Digestion.pdf
- Short Description:
- Protocol from the NPC Access site for the in-solution digestion of proteins using various different enzymes (Trypsin, Lys-C, Lys-N).
- Submitted On:
- 12 Aug 2011
- File Size:
- 145.32 Kb
- Downloads:
- 144
PRIME-XS Protocol VIB In-Gel Stable Isotope Labeling (ISIL)
- Short Description:
- This protocol is used to mass tag primary amines of gel-separated proteins. This mass tagging occurs also at protein N-termini (alpha-amines) and c...
- Submitted On:
- 13 Mar 2012
- File Size:
- 87.00 Kb
- Downloads:
- 22
PRIME-XS Protocol VIB N-propionylation of endoLys-C generated peptides
- Short Description:
- This protocol is used for post-metabolic labeling of peptides generated by endoproteinase Lys-C for differential proteome analysis.
- Submitted On:
- 13 Mar 2012
- File Size:
- 139.36 Kb
- Downloads:
- 34
PRIME-XS Protocol VIB Enrichment of tryptic N-terminal peptides by COFRADIC
- Short Description:
- This protocol is used for enriching N-terminal peptides from whole proteome digests using the positional proteomics N-terminal COFRADIC technology.
- Submitted On:
- 13 Mar 2012
- File Size:
- 155.95 Kb
- Downloads:
- 28
PRIME-XS protocol VIB Enrichment of tryptic N-terminal peptides through SCX
- Short Description:
- Strong cation exchange chromatography at acidic pH is used to enrich for protein N-terminal peptides that are proxies for N-terminal protein modifi...
- Submitted On:
- 13 Mar 2012
- File Size:
- 132.00 Kb
- Downloads:
- 29
PRIME-XS Protocol VIB Enrichment of peptides containing nitrated tyrosine residues by COFRADIC
- Short Description:
- Nitration of tyrosine residues readily occurs when cells are under conditions of oxidative or nitrosative stress. This protocol is based on the COF...
- Submitted On:
- 20 Mar 2012
- File Size:
- 135.14 Kb
- Downloads:
- 15
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